Determination
of chlorophyll and anthocyanins can be done based on Lambert-Beer’s Law. . The molar absorbency of
each anthocyanin may be slightly different depending on the standard chosen as
a mixture of major anthocyanins may be found present in most of the fruits. The
total anthocyanin content is measured as cyanidin-3-glucoside due to its wide
usage for similar assays and availability. Anthocyanin possesses a molecular
weight of 449 and molar absorbency of 26900 at absorbance wavelength of 510 nm
at pH 1.
The
amount of chlorophyll can be determined by using a spectrophotometer. The
absorbance is proportional to the concentration of chlorophyll presents in the
sample [9]. Chlorophyll possesses a molecular weight
of 893.49 gmol-1 and molar absorbency of 105 M-1 cm-1
at absorbance wavelength of 663nm.
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